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Promega deadend fluorometric tunel system
Deadend Fluorometric Tunel System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/deadend+fluorometric+tunel+system/deadend+fluorometric+tunel+system/pm40603745-392-10-22
Average 90 stars, based on 1 article reviews
deadend fluorometric tunel system - by Bioz Stars, 2026-09
90/100 stars

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TUNEL Assay:

Article Title: METTL3-dependent m6A RNA methylation regulates transposable elements and represses human naïve pluripotency through transposable element-derived enhancers.
Article Snippet: .. The DNase I-TUNEL assay was conducted using the DeadEnd Fluorometric TUNEL System (Promega) following the manufacturer’s protocols. .. Cells were treated with 1 U / ml of DNase I (Thermo Scientific) for 5 min at 37 ◦C before rTdT labeling.

Article Title: RIPK1 in Diffuse Glioma Pathology: From Prognosis Marker to Potential Therapeutic Target.
Article Snippet: Absorbance at 595 nm was read in an automated plate spectrophotometer (TECAN Infinite 200 PRO, Crailsheim, Germany). .. Apoptotic cells were detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the DeadEnd Fluorometric TUNEL System (Promega, Madison, WI, USA) and DNA was stained with DAPI. ..

Article Title: Spinal Cord Organoids from Human Amniotic Fluid iPSC Recapitulate the Diversity of Cell Phenotypes During Fetal Neural Tube Morphogenesis.
Article Snippet: Myelomeningocele (MMC) is a severe form of spina bifida associated with substantial neurologic morbidity.. In vitro modeling systems of human spinal cord development may help to elucidate the underlying pathophysiology of the MMC spinal cord.. To that end, we developed spinal cord organoids (SCO), defined as self-organized, three-dimensional clusters of spinal tissue, that were derived from human amniotic fluid–induced pluripotent stem cells.

Article Title: Naphthenic Acid Fraction Components-Induced Metabolic and Mitochondrial Alterations in Rat Hepatoma Cells: Monitoring Metabolic Reprogramming with Tryptophan-Kynurenine Ratio.
Article Snippet: Briefly, cells were seeded in 96-well plates and allowed to adhere for 24 h, after which the cells were exposed to the vehicle control or NAFCs [0.73, 14.7, and 73.4 mg/L] for 24 and 48 h. Fluorescence measurements were taken for JC-1 aggregates and monomers (Ex/Em = 475/590, 530 nm) on a Synergy H1 Hybrid Reader (Agilent Technologies, Inc.). .. To determine if NAFC exposure caused increased cell death via apoptosis, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed with a DeadEnd fluorometric TUNEL system (Promega) following the manufacturer’s protocol [35]. ..

Article Title: Optimizing the Surface Functionalization of Peptide–MXene Nanoplatforms to Amplify Tumor-Targeting Efficiency and Photothermal Therapy
Article Snippet: .. Similarly, the TUNEL assay was performed as per the user guidelines mentioned in the DeadEnd Fluorometric TUNEL system (Promega Korea). ..

Article Title: Peptidylarginine Deiminase 4 Deficiency Suppresses Neutrophil Extracellular Trap Formation and Ameliorates Elastase-Induced Emphysema in Mouse Lung
Article Snippet: The samples were observed using a low-vacuum SEM TM3030 (Hitachi HighTech Corporation, Tokyo, Japan). .. To evaluate apoptosis, we performed TUNEL staining on day 14 using the DeadEnd Fluorometric TUNEL System (Promega, Fitchburg, WI, USA, cat. G3250) following the protocol provided by the manufacturer. .. We used rabbit anti-Prosurfactant Protein C antibody (1:1170; Abcam, Cambridge, UK, cat. ab90716), anti-RAGE antibody (1:4000; Abcam, Cambridge, UK, cat. ab228861), anti-CD31 antibody (1:2000; Abcam, Cambridge, UK, cat. ab182981), and alpaca anti-rabbit IgG Alexa Fluor 594 (1:500; Jackson ImmunoReseach, West Grove, PA, USA, cat. 611-585-215) for co-staining.

Article Title: Naphthenic Acid Fraction Components-Induced Metabolic and Mitochondrial Alterations in Rat Hepatoma Cells: Monitoring Metabolic Reprogramming with Tryptophan–Kynurenine Ratio
Article Snippet: Briefly, cells were seeded in 96-well plates and allowed to adhere for 24 h, after which the cells were exposed to the vehicle control or NAFCs [0.73, 14.7, and 73.4 mg/L] for 24 and 48 h. Fluorescence measurements were taken for JC-1 aggregates and monomers (Ex/Em = 475/590, 530 nm) on a Synergy H1 Hybrid Reader (Agilent Technologies, Inc.). .. To determine if NAFC exposure caused increased cell death via apoptosis, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed with a DeadEnd fluorometric TUNEL system (Promega) following the manufacturer’s protocol [ ]. ..

other:

Article Title: Jawbone-like organoids generated from human pluripotent stem cells.
Article Snippet: To identify apoptotic cells, sectioned samples were subjected to terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining (DeadEnd Fluorometric TUNEL System; Promega).

Staining:

Article Title: RIPK1 in Diffuse Glioma Pathology: From Prognosis Marker to Potential Therapeutic Target.
Article Snippet: Absorbance at 595 nm was read in an automated plate spectrophotometer (TECAN Infinite 200 PRO, Crailsheim, Germany). .. Apoptotic cells were detected using terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) using the DeadEnd Fluorometric TUNEL System (Promega, Madison, WI, USA) and DNA was stained with DAPI. ..

Article Title: Spinal Cord Organoids from Human Amniotic Fluid iPSC Recapitulate the Diversity of Cell Phenotypes During Fetal Neural Tube Morphogenesis.
Article Snippet: Myelomeningocele (MMC) is a severe form of spina bifida associated with substantial neurologic morbidity.. In vitro modeling systems of human spinal cord development may help to elucidate the underlying pathophysiology of the MMC spinal cord.. To that end, we developed spinal cord organoids (SCO), defined as self-organized, three-dimensional clusters of spinal tissue, that were derived from human amniotic fluid–induced pluripotent stem cells.

Article Title: Naphthenic Acid Fraction Components-Induced Metabolic and Mitochondrial Alterations in Rat Hepatoma Cells: Monitoring Metabolic Reprogramming with Tryptophan-Kynurenine Ratio.
Article Snippet: Briefly, cells were seeded in 96-well plates and allowed to adhere for 24 h, after which the cells were exposed to the vehicle control or NAFCs [0.73, 14.7, and 73.4 mg/L] for 24 and 48 h. Fluorescence measurements were taken for JC-1 aggregates and monomers (Ex/Em = 475/590, 530 nm) on a Synergy H1 Hybrid Reader (Agilent Technologies, Inc.). .. To determine if NAFC exposure caused increased cell death via apoptosis, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed with a DeadEnd fluorometric TUNEL system (Promega) following the manufacturer’s protocol [35]. ..

Article Title: Peptidylarginine Deiminase 4 Deficiency Suppresses Neutrophil Extracellular Trap Formation and Ameliorates Elastase-Induced Emphysema in Mouse Lung
Article Snippet: The samples were observed using a low-vacuum SEM TM3030 (Hitachi HighTech Corporation, Tokyo, Japan). .. To evaluate apoptosis, we performed TUNEL staining on day 14 using the DeadEnd Fluorometric TUNEL System (Promega, Fitchburg, WI, USA, cat. G3250) following the protocol provided by the manufacturer. .. We used rabbit anti-Prosurfactant Protein C antibody (1:1170; Abcam, Cambridge, UK, cat. ab90716), anti-RAGE antibody (1:4000; Abcam, Cambridge, UK, cat. ab228861), anti-CD31 antibody (1:2000; Abcam, Cambridge, UK, cat. ab182981), and alpaca anti-rabbit IgG Alexa Fluor 594 (1:500; Jackson ImmunoReseach, West Grove, PA, USA, cat. 611-585-215) for co-staining.

Article Title: Naphthenic Acid Fraction Components-Induced Metabolic and Mitochondrial Alterations in Rat Hepatoma Cells: Monitoring Metabolic Reprogramming with Tryptophan–Kynurenine Ratio
Article Snippet: Briefly, cells were seeded in 96-well plates and allowed to adhere for 24 h, after which the cells were exposed to the vehicle control or NAFCs [0.73, 14.7, and 73.4 mg/L] for 24 and 48 h. Fluorescence measurements were taken for JC-1 aggregates and monomers (Ex/Em = 475/590, 530 nm) on a Synergy H1 Hybrid Reader (Agilent Technologies, Inc.). .. To determine if NAFC exposure caused increased cell death via apoptosis, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was performed with a DeadEnd fluorometric TUNEL system (Promega) following the manufacturer’s protocol [ ]. ..



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